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長波長発光を示す生物発光気質の開発およびその性質
https://uec.repo.nii.ac.jp/records/8657
https://uec.repo.nii.ac.jp/records/86570de4df2d-d6b1-47c2-abe8-59e4e1d8c94c
名前 / ファイル | ライセンス | アクション |
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1343001_Thesis.pdf (7.2 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2018-04-04 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 長波長発光を示す生物発光気質の開発およびその性質 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Development and Properties of Red-shifted Bioluminescent Substrate | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
木山, 正啓
× 木山, 正啓 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Bioluminescence reactions of fireflies and marine organisms are widely used for bioanalysis including in vivo bioluminescence imaging. In vivo bioluminescence imaging, wavelengths in the red to near-infrared region are highly advantageous for tissue permeability. However, many bioluminescent substances emit at shorter wavelengths (λ ≤ 600nm). Recently, we developed a red firefly bioluminescent substrate (AkaLumine, λmax= ca. 670 nm), getting good results with in vivo bioluminescence imaging. However, the emission intensity is not sufficient. In this paper, we synthesized AkaLumine analogues introducing a cyclic amino group, generating high brightness while maintaining red-light-emission. On the other hand, red-light-emission (λ > 600nm) of the marine bioluminescent substance “coelenterazine (CTZ)” without energy transfer has not been achieved. We found that CTZ analogues with multi-olefines at the C-6 position of imidazopyrazinone produced red-light emission (λmax= 664 nm), and clarified their bioluminescence properties. 赤色ホタル生物発光基質AkaLumine(λmax = ca. 670 nm)にいくつかの環状アミノ基を導入することで、長波長発光を維持しつつ高輝度化を達成した。マウスを用いたin vivo発光測定により、長波長発光および高輝度化した発光基質の有効性を確かめた。また、海洋生物発光基質セレンテラジンのイミダゾピラジノン骨格C’6位をオレフィン化し、オレフィン数による発光波長の制御が可能であることを見出した。これらの基質の1つで、セレテンラジン系発光基質としては世界最長波長を達成した(λmax = 664nm)。 |
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学位名 | ||||||
学位名 | 博士(理学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 12612 | |||||
学位授与機関名 | 電気通信大学 | |||||
学位授与年度 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 2017 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2018-03-23 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第934号 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
専攻 | ||||||
情報理工学研究科 | ||||||
専攻 | ||||||
先進理工学専攻 | ||||||
学術成果タイプ | ||||||
博士学位論文 |