{"created":"2023-05-15T08:43:37.416477+00:00","id":8657,"links":{},"metadata":{"_buckets":{"deposit":"883202bb-9e4b-43f8-bd57-3d44c58ff6ea"},"_deposit":{"created_by":13,"id":"8657","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"8657"},"status":"published"},"_oai":{"id":"oai:uec.repo.nii.ac.jp:00008657","sets":["9:178"]},"author_link":["23493"],"control_number":"8657","item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2018-03-23"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"電気通信大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"12612","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2017","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Bioluminescence reactions of fireflies and marine organisms are widely used \nfor bioanalysis including in vivo  bioluminescence imaging. In vivo bioluminescence imaging, wavelengths in the red to near-infrared region are highly advantageous for tissue permeability. However, many bioluminescent substances emit at shorter wavelengths (λ ≤ 600nm). Recently, we developed a red firefly bioluminescent substrate (AkaLumine, λmax= ca. 670 nm), getting good results with in vivo bioluminescence imaging. However, the emission intensity is not sufficient. In this paper, we synthesized AkaLumine analogues introducing a cyclic amino group, generating high brightness while maintaining red-light-emission. On the other hand, red-light-emission (λ > 600nm) of the marine bioluminescent substance “coelenterazine (CTZ)” without energy transfer has not been achieved. We found that CTZ analogues with multi-olefines at the C-6 position of imidazopyrazinone produced red-light emission (λmax= 664 nm), and clarified their bioluminescence properties. \n\n  赤色ホタル生物発光基質AkaLumine(λmax = ca. 670 nm)にいくつかの環状アミノ基を導入することで、長波長発光を維持しつつ高輝度化を達成した。マウスを用いたin vivo発光測定により、長波長発光および高輝度化した発光基質の有効性を確かめた。また、海洋生物発光基質セレンテラジンのイミダゾピラジノン骨格C’６位をオレフィン化し、オレフィン数による発光波長の制御が可能であることを見出した。これらの基質の１つで、セレテンラジン系発光基質としては世界最長波長を達成した(λmax = 664nm)。","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第934号"}]},"item_10006_text_22":{"attribute_name":"専攻","attribute_value_mlt":[{"subitem_text_value":"情報理工学研究科"},{"subitem_text_value":"先進理工学専攻"}]},"item_10006_text_23":{"attribute_name":"学術成果タイプ","attribute_value_mlt":[{"subitem_text_value":"博士学位論文"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木山, 正啓","creatorNameLang":"ja"},{"creatorName":"キヤマ, マサヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-04-04"}],"displaytype":"detail","filename":"1343001_Thesis.pdf","filesize":[{"value":"7.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"1343001_Thesis.pdf","url":"https://uec.repo.nii.ac.jp/record/8657/files/1343001_Thesis.pdf"},"version_id":"b44d4d9a-16c0-4956-ab17-c770cbb98f34"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"長波長発光を示す生物発光気質の開発およびその性質","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"長波長発光を示す生物発光気質の開発およびその性質","subitem_title_language":"ja"},{"subitem_title":"Development and Properties of Red-shifted Bioluminescent Substrate","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"13","path":["178"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-04-04"},"publish_date":"2018-04-04","publish_status":"0","recid":"8657","relation_version_is_last":true,"title":["長波長発光を示す生物発光気質の開発およびその性質"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-07-07T06:23:54.392549+00:00"}