@phdthesis{oai:uec.repo.nii.ac.jp:00008657,
 author = {木山, 正啓},
 month = {2018-04-04},
 note = {2017, Bioluminescence reactions of fireflies and marine organisms are widely used 
for bioanalysis including in vivo  bioluminescence imaging. In vivo bioluminescence imaging, wavelengths in the red to near-infrared region are highly advantageous for tissue permeability. However, many bioluminescent substances emit at shorter wavelengths (λ ≤ 600nm). Recently, we developed a red firefly bioluminescent substrate (AkaLumine, λmax= ca. 670 nm), getting good results with in vivo bioluminescence imaging. However, the emission intensity is not sufficient. In this paper, we synthesized AkaLumine analogues introducing a cyclic amino group, generating high brightness while maintaining red-light-emission. On the other hand, red-light-emission (λ > 600nm) of the marine bioluminescent substance “coelenterazine (CTZ)” without energy transfer has not been achieved. We found that CTZ analogues with multi-olefines at the C-6 position of imidazopyrazinone produced red-light emission (λmax= 664 nm), and clarified their bioluminescence properties. 

  赤色ホタル生物発光基質AkaLumine(λmax = ca. 670 nm)にいくつかの環状アミノ基を導入することで、長波長発光を維持しつつ高輝度化を達成した。マウスを用いたin vivo発光測定により、長波長発光および高輝度化した発光基質の有効性を確かめた。また、海洋生物発光基質セレンテラジンのイミダゾピラジノン骨格C’６位をオレフィン化し、オレフィン数による発光波長の制御が可能であることを見出した。これらの基質の１つで、セレテンラジン系発光基質としては世界最長波長を達成した(λmax = 664nm)。},
 school = {電気通信大学},
 title = {長波長発光を示す生物発光気質の開発およびその性質},
 year = {},
 yomi = {キヤマ, マサヒロ}
}