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Chemical labelling for visualizing native AMPA receptors in live neurons
https://uec.repo.nii.ac.jp/records/9072
https://uec.repo.nii.ac.jp/records/907205536e11-6203-4e8b-9b2b-6f3672ccb3f2
名前 / ファイル | ライセンス | アクション |
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ncomms14850 (2.5 MB)
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Creative Commons Attribution 4.0 International License (CC BY)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-04-05 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Chemical labelling for visualizing native AMPA receptors in live neurons | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Wakayama, Sho
× Wakayama, Sho× Kiyonaka, Shigeki× Arai, Itaru× Kakegawa, Wataru× Matsuda, Shinji× Ibata, Keiji× Nemoto, Yuri L.× Kusumi, Akihiro× Yuzaki, Michisuke× Hamachi, Itaru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The location and number of neurotransmitter receptors are dynamically regulated at postsynaptic sites. However, currently available methods for visualizing receptor trafficking require the introduction of genetically engineered receptors into neurons, which can disrupt the normal functioning and processing of the original receptor. Here we report a powerful method for visualizing native α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors (AMPARs) which are essential for cognitive functions without any genetic manipulation. This is based on a covalent chemical labelling strategy driven by selective ligand-protein recognition to tether small fluorophores to AMPARs using chemical AMPAR modification (CAM) reagents. The high penetrability of CAM reagents enables visualization of native AMPARs deep in brain tissues without affecting receptor function. Moreover, CAM reagents are used to characterize the diffusion dynamics of endogenous AMPARs in both cultured neurons and hippocampal slices. This method will help clarify the involvement of AMPAR trafficking in various neuropsychiatric and neurodevelopmental disorders. | |||||
書誌情報 |
en : Nature Communications 巻 8, p. 14850, 発行日 2017-04-07 |
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出版者 | ||||||
出版者 | Nature Research | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 2041-1723 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/ncomms14850 | |||||
権利 | ||||||
権利情報 | © The Author(s) 2017 | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1038/ncomms14850 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |