{"created":"2023-05-15T08:38:09.816985+00:00","id":944,"links":{},"metadata":{"_buckets":{"deposit":"04bfcc8b-3427-4708-8830-8778cfd20874"},"_deposit":{"created_by":3,"id":"944","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"944"},"status":"published"},"_oai":{"id":"oai:uec.repo.nii.ac.jp:00000944","sets":["9:19"]},"author_link":["6768"],"control_number":"944","item_10006_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"T7ファージ提示ペプチドのチオエーテル化を基盤としたハイブリッド分子ライブラリーの作製","subitem_alternative_title_language":"ja"}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-24"}]},"item_10006_degree_grantor_9":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"電気通信大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"12612","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（工学）"}]},"item_10006_description_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_description":"2013","subitem_description_type":"Other"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available pre-made phage libraries allow us to take screening in the easiest way. On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions. This chapter focuses on what should be born in mind for beginners using commercially available cloning kits (i.e., Ph.D. with type 3 vector and T7Select systems for M13 and T7 phage, respectively). In the M13 system, proline or basic amino acids (especially, Arg) should be avoided at the N-terminus of peptide fused to gp3. In both systems, peptides containing odd number(s) of cysteine(s) should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第767号"}]},"item_10006_version_type_18":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福永, 圭佑","creatorNameLang":"ja"},{"creatorName":"フクナガ, ケイスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Fukunaga, Keisuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-09-15"}],"displaytype":"detail","filename":"9000000746.pdf","filesize":[{"value":"11.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"9000000746.pdf","url":"https://uec.repo.nii.ac.jp/record/944/files/9000000746.pdf"},"version_id":"22bbef97-9337-460b-bcfa-80f08d62a847"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Construction of hybrid molecule libraries based on thioetherification of T7 phage-displayed peptides","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Construction of hybrid molecule libraries based on thioetherification of T7 phage-displayed peptides","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"3","path":["19"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2014-03-24"},"publish_date":"2014-03-24","publish_status":"0","recid":"944","relation_version_is_last":true,"title":["Construction of hybrid molecule libraries based on thioetherification of T7 phage-displayed peptides"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-02-13T01:32:36.227351+00:00"}