@phdthesis{oai:uec.repo.nii.ac.jp:00000944,
 author = {福永, 圭佑 and Fukunaga, Keisuke},
 month = {2016-09-15},
 note = {2013, Phage display technology is undoubtedly a powerful tool for affinity selection of target-specific peptide. Commercially available pre-made phage libraries allow us to take screening in the easiest way. On the other hand, construction of a custom phage library seems to be inaccessible, because several practical tips are absent in instructions. This chapter focuses on what should be born in mind for beginners using commercially available cloning kits (i.e., Ph.D. with type 3 vector and T7Select systems for M13 and T7 phage, respectively). In the M13 system, proline or basic amino acids (especially, Arg) should be avoided at the N-terminus of peptide fused to gp3. In both systems, peptides containing odd number(s) of cysteine(s) should be designed with caution. Also, DNA sequencing of a constructed library before biopanning is highly recommended for finding unexpected bias.},
 school = {電気通信大学},
 title = {Construction of hybrid molecule libraries based on thioetherification of T7 phage-displayed peptides},
 year = {},
 yomi = {フクナガ, ケイスケ}
}