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Subunit-dependent and subunit-independent rules of AMPA receptor trafficking during chemical long-term depression in hippocampal neurons
https://uec.repo.nii.ac.jp/records/10053
https://uec.repo.nii.ac.jp/records/1005341e05809-b907-49ef-8291-d4573c648828
名前 / ファイル | ライセンス | アクション |
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Final QC full formatted PDF Matsuda & Yuzaki 20210705.pdf (3.6 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-09-27 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Subunit-dependent and subunit-independent rules of AMPA receptor trafficking during chemical long-term depression in hippocampal neurons | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題 | AMPA | |||||
キーワード | ||||||
言語 | en | |||||
主題 | adaptor protein | |||||
キーワード | ||||||
言語 | en | |||||
主題 | endocytosis | |||||
キーワード | ||||||
言語 | en | |||||
主題 | ionotropic glutamate receptor | |||||
キーワード | ||||||
言語 | en | |||||
主題 | LTP | |||||
キーワード | ||||||
言語 | en | |||||
主題 | LTD | |||||
キーワード | ||||||
言語 | en | |||||
主題 | neuron | |||||
キーワード | ||||||
言語 | en | |||||
主題 | phosphorylation | |||||
キーワード | ||||||
言語 | en | |||||
主題 | receptor recycling | |||||
キーワード | ||||||
言語 | en | |||||
主題 | synaptic plasticity | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Matsuda, Shinji
× Matsuda, Shinji× Yuzaki, Michisuke |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Long-term potentiation (LTP) and long-term depression (LTD) of excitatory neurotransmission are believed to be the neuronal basis of learning and memory. Both processes are primarily mediated by neuronal activity–induced transport of postsynaptic AMPA-type glutamate receptors (AMPARs). While AMPAR subunits and their specific phosphorylation sites mediate differential AMPAR trafficking, LTP and LTD could also occur in a subunit-independent manner. Thus, it remains unclear whether and how certain AMPAR subunits with phosphorylation sites are preferentially recruited to or removed from synapses during LTP and LTD. Using immunoblot and immunocytochemical analysis, we show that phosphomimetic mutations of the membrane-proximal region (MPR) in GluA1 AMPAR subunits affect the subunit-dependent endosomal transport of AMPARs during chemical LTD. AP-2 and AP-3, adaptor protein complexes necessary for clathrin-mediated endocytosis and late endosomal/lysosomal trafficking, respectively, are reported to be recruited to AMPARs by binding to the AMPAR auxiliary subunit, stargazin (STG), in an AMPAR subunit–independent manner. However, the association of AP-3, but not AP-2, with STG was indirectly inhibited by the phosphomimetic mutation in the MPR of GluA1. Thus, although AMPARs containing the phosphomimetic mutation at the MPR of GluA1 were endocytosed by a chemical LTD-inducing stimulus, they were quickly recycled back to the cell surface in hippocampal neurons. These results could explain how the phosphorylation status of GluA1-MPR plays a dominant role in subunit-independent STG-mediated AMPAR trafficking during LTD. | |||||
書誌情報 |
en : Journal of Biological Chemistry 巻 297, 号 2, p. 100949, 発行日 2021-08 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00219258 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jbc.2021.100949 | |||||
権利 | ||||||
権利情報 | (c) 2021 THE AUTHORS. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | |||||
関連サイト | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/j.jbc.2021.100949 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |